الوصف
Human Chitinase-3-like Protein 1(YKL-40 or CHI3L1) ELISA Kit@REFERENCES
The kit is for the quantitative level of YKL-40/CHI3L1 in the sample, adopt purified Human YKL-40/CHI3L1 to coat microtiter
plate, make solid-phase antibody, then add YKL-40/CHI3L1 to wells, Combine YKL-40/CHI3L1 antibody with labeled HRP to
form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB
substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and
the color change is measured at a wavelength of 450 nm. The concentration of YKL-40/CHI3L1 in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
المراجعات
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