الوصف
Human Glutamate?Glu?ELISA Kit
The kit is for the quantitative level of Glu in the sample, adopt purified Human Glu
antibody to coat microtiter plate, make solid-phase antibody, then add samples or
standards to wells with a labeled antibody specific to Glu, then add labeled HRP to the
well. After washing completely, add TMB substrate solution, TMB substrate
becomes blue color in wells that contains antibody – antigen – enzyme-antibody
complex, reaction is terminated by the addition of a stop solution and the color
change is measured at a wavelength of 450 nm. The concentration of Glu in the
samples is then determined by comparing the O.D. of the samples to the standard
curve.
المراجعات
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