الوصف
Human cyclooxygenase-1(COX-1) ELISA Kit
The kit assay Human COX-1 level in the sample，use Purified Human COX-1 antibody to coat microtiter plate wells,
make solid-phase antibody, then add COX-1 to wells, Combined COX-1 which With HRP labeled , become antibody – antigen
– enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At
HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm. The concentration of COX-1 in the samples is then determined by
comparing the O.D. of the samples to the standard curve.
المراجعات
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