الوصف
Human YKL-40 ELISA Kit ELISA Kit
The kit assay Human YKL-40 level in the sample，use Purified Human YKL-40 antibody to coat microtiter plate wells,
make solid-phase antibody, then add YKL-40 to wells, Combined YKL-40 antibody which With HRP labeled ， become
antibody – antigen – enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate
becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of YKL-40 in the samples is
then determined by comparing the O.D. of the samples to the standard curve.
المراجعات
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