الوصف
Human Linolenic acid?LA?ELISA Kit
The kit is for the quantitative level of LA in the sample, adopt purified Human LA to coat
microtiter plate, make solid-phase antibody, then add samples or standards to wells
with a labeled antibody specific to LA, then add labeled HRP to the well. After washing
completely, add TMB substrate solution, TMB substrate becomes blue color in wells
that contains antibody – antigen – enzyme-antibody complex, reaction is terminated
by the addition of a stop solution and the color change is measured at a wavelength of
450 nm. The concentration of LA in the samples is then determined by comparing the
O.D. of the samples to the standard curve.
المراجعات
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