الوصف
Bovine oxytocin (OT) ELISA Kit
The kit is for the quantitative level of OT in the sample, adopt purified Bovine OT to coat microtiter plate, make solid-phase
antibody, then add OT to wells, Combine OT antibody with labeled HRP to form antibody-antigen -enzyme-antibody
complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP
enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of OT in the samples is then determined by comparing the O.D. of the samples to
the standard curve.
المراجعات
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