الوصف
Human 1?3-Disphosphoglycerate (1,3-DPG) ELISA Kit
The kit is for the quantitative level of 1,3-DPG in the sample, adopt purified Human 1,3-DPG to coat microtiter plate, make
solid-phase antibody, then add 1,3-DPG to wells, Combine 1,3-DPG antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of 1,3-DPG in the samples is then determined by comparing the O.D. of the
samples to the standard curve.
المراجعات
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