الوصف
Human Epidermal growth factor(EGF) ELISA Kit
The kit is for the quantitative level of EGF in the sample, adopt purified Human EGF to coat microtiter plate, make
solid-phase antibody, then add EGF to wells, Combine EGF antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue
color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is
measured at a wavelength of 450 nm. The concentration of EGF in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
المراجعات
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