الوصف
Mouse pituitary adenylate cyclase activating polypeptide,PACAP ELISAkit
The kit is for the quantitative level of PACAP in the sample, adopt purified Rat PACAP to coat microtiter plate, make
solid-phase antibody, then add PACAP to wells, Combine PACAP antibody with labeled HRP to form
antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB
substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution
and the color change is measured at a wavelength of 450 nm. The concentration of PACAP in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
المراجعات
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