الوصف
Rat theophylline ELISA Kit
The kit is for the quantitative level of theophylline in the sample, adopt purified Rat
theophylline antibody to coat microtiter plate, make solid-phase antibody, then add
samples or standards to wells with a labeled antibody specific to theophylline, then add
labeled HRP to the well. After washing completely, add TMB substrate solution, TMB
substrate becomes blue color in wells that contains antibody – antigen –
enzyme-antibody complex, reaction is terminated by the addition of a stop solution
and the color change is measured at a wavelength of 450 nm. The concentration of
theophylline in the samples is then determined by comparing the O.D. of the samples
to the standard curve.
المراجعات
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