الوصف
Human Pulmonary surfatcant-associated protein A,SP-A ELISA Kit
The kit is for the quantitative level of SP-A in the sample, adopt purified human SP-A to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to SP-A, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody – antigen – enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of SP-A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
المراجعات
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