الوصف
Human Pyruvate Kinase,M2-PK ELISA Kit
The kit is for the quantitative level of M2-PK in the sample, adopt purified Human M2-PK to coat microtiter plate, make
solid-phase antibody, then add M2-PK to wells, Combine M2-PK antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of M2-PK in the samples is then determined by comparing the O.D. of the
samples to the standard curve.
المراجعات
لا توجد مراجعات بعد.