الوصف
Human tissue inhibitor of metal protease 1,TIMP-1 ELISA Kit
The kit is for the quantitative level of TIMP-1 in the sample, adopt purified human TIMP-1 to coat microtiter plate,
make solid-phase antibody, then add TIMP-1 to wells, Combine TIMP-1 antibody with labeled HRP to form
antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB
substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution
and the color change is measured at a wavelength of 450 nm. The concentration of TIMP-1 in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
المراجعات
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