الوصف
Human Tuberculin ELISA Kit
The kit is for the quantitative level of Tuberculin in the sample, adopt purified Human
Tuberculin antibody to coat microtiter plate, make solid-phase antibody, then add
samples or standards to wells with a labeled antibody specific to Tuberculin, then add
labeled HRP to the well. After washing completely, add TMB substrate solution, TMB
substrate becomes blue color in wells that contains antibody – antigen –
enzyme-antibody complex, reaction is terminated by the addition of a stop solution
and the color change is measured at a wavelength of 450 nm. The concentration of
Tuberculin in the samples is then determined by comparing the O.D. of the samples to
the standard curve.
المراجعات
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